Working slice electrophysiology and calcium imaging

In 1991, the Feldman lab demonstrated that a brainstem slice containing the breathing rhythm generator, the preBötC, continues to produce respiratory rhythms when explanted in vitro. Surrounded by talented electrophysiology labs at UCSF and with advice from experts in the field (thanks Kaiwen!) we recently have established this as a new technique in the lab. We are excited to begin molecularly defining neurons with interesting and exciting activity during the breathing cycle by coupling calcium imaging and electrophysiology.